Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2548081959', 'doi': 'https://doi.org/10.1016/j.mimet.2016.10.022', 'title': 'A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining', 'display_name': 'A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining', 'publication_year': 2016, 'publication_date': '2016-11-01', 'ids': {'openalex': 'https://openalex.org/W2548081959', 'doi': 'https://doi.org/10.1016/j.mimet.2016.10.022', 'mag': '2548081959', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/27810378'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.mimet.2016.10.022', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S24124513', 'display_name': 'Journal of Microbiological Methods', 'issn_l': '0167-7012', 'issn': ['0167-7012', '1872-8359'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5009834109', 'display_name': 'Alīna Neščerecka', 'orcid': 'https://orcid.org/0000-0003-4689-2545'}, 'institutions': [{'id': 'https://openalex.org/I63664421', 'display_name': 'Swiss Federal Institute of Aquatic Science and Technology', 'ror': 'https://ror.org/00pc48d59', 'country_code': 'CH', 'type': 'facility', 'lineage': ['https://openalex.org/I2799323385', 'https://openalex.org/I63664421']}, {'id': 'https://openalex.org/I201787326', 'display_name': 'Riga Technical University', 'ror': 'https://ror.org/00twb6c09', 'country_code': 'LV', 'type': 'education', 'lineage': ['https://openalex.org/I201787326']}], 'countries': ['CH', 'LV'], 'is_corresponding': False, 'raw_author_name': 'Alina Nescerecka', 'raw_affiliation_strings': ['Eawag, Swiss Federal Institute for Aquatic Science and Technology, Überlandstr. 133, CH-8600 Dübendorf, Switzerland', 'Riga Technical University, Ķīpsalas Str. 6A, LV-1048, Riga, Latvia'], 'affiliations': [{'raw_affiliation_string': 'Eawag, Swiss Federal Institute for Aquatic Science and Technology, Überlandstr. 133, CH-8600 Dübendorf, Switzerland', 'institution_ids': ['https://openalex.org/I63664421']}, {'raw_affiliation_string': 'Riga Technical University, Ķīpsalas Str. 6A, LV-1048, Riga, Latvia', 'institution_ids': ['https://openalex.org/I201787326']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5016112403', 'display_name': 'Frederik Hammes', 'orcid': 'https://orcid.org/0000-0002-4596-4917'}, 'institutions': [{'id': 'https://openalex.org/I63664421', 'display_name': 'Swiss Federal Institute of Aquatic Science and Technology', 'ror': 'https://ror.org/00pc48d59', 'country_code': 'CH', 'type': 'facility', 'lineage': ['https://openalex.org/I2799323385', 'https://openalex.org/I63664421']}], 'countries': ['CH'], 'is_corresponding': True, 'raw_author_name': 'Frederik Hammes', 'raw_affiliation_strings': ['Eawag, Swiss Federal Institute for Aquatic Science and Technology, Überlandstr. 133, CH-8600 Dübendorf, Switzerland'], 'affiliations': [{'raw_affiliation_string': 'Eawag, Swiss Federal Institute for Aquatic Science and Technology, Überlandstr. 133, CH-8600 Dübendorf, Switzerland', 'institution_ids': ['https://openalex.org/I63664421']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5031882671', 'display_name': 'Tālis Juhna', 'orcid': 'https://orcid.org/0000-0001-5985-704X'}, 'institutions': [{'id': 'https://openalex.org/I201787326', 'display_name': 'Riga Technical University', 'ror': 'https://ror.org/00twb6c09', 'country_code': 'LV', 'type': 'education', 'lineage': ['https://openalex.org/I201787326']}], 'countries': ['LV'], 'is_corresponding': False, 'raw_author_name': 'Talis Juhna', 'raw_affiliation_strings': ['Riga Technical University, Ķīpsalas Str. 6A, LV-1048, Riga, Latvia'], 'affiliations': [{'raw_affiliation_string': 'Riga Technical University, Ķīpsalas Str. 6A, LV-1048, Riga, Latvia', 'institution_ids': ['https://openalex.org/I201787326']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': ['https://openalex.org/A5016112403'], 'corresponding_institution_ids': ['https://openalex.org/I63664421'], 'apc_list': {'value': 2850, 'currency': 'USD', 'value_usd': 2850, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 2.301, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 80, 'citation_normalized_percentile': {'value': 0.841983, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 97, 'max': 98}, 'biblio': {'volume': '131', 'issue': None, 'first_page': '172', 'last_page': '180'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11255', 'display_name': 'Microfluidic and Bio-sensing Technologies', 'score': 0.9778, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11255', 'display_name': 'Microfluidic and Bio-sensing Technologies', 'score': 0.9778, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T12859', 'display_name': 'Cell Image Analysis Techniques', 'score': 0.9748, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11393', 'display_name': 'Biosensors and Analytical Detection', 'score': 0.9748, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/propidium-iodide', 'display_name': 'Propidium iodide', 'score': 0.89745486}, {'id': 'https://openalex.org/keywords/sybr-green-i', 'display_name': 'SYBR Green I', 'score': 0.5052543}], 'concepts': [{'id': 'https://openalex.org/C74864618', 'wikidata': 'https://www.wikidata.org/wiki/Q2332446', 'display_name': 'Staining', 'level': 2, 'score': 0.94188726}, {'id': 'https://openalex.org/C2775934118', 'wikidata': 'https://www.wikidata.org/wiki/Q424169', 'display_name': 'Propidium iodide', 'level': 4, 'score': 0.89745486}, {'id': 'https://openalex.org/C553184892', 'wikidata': 'https://www.wikidata.org/wiki/Q1141429', 'display_name': 'Flow cytometry', 'level': 2, 'score': 0.65929395}, {'id': 'https://openalex.org/C2780968160', 'wikidata': 'https://www.wikidata.org/wiki/Q423692', 'display_name': 'SYBR Green I', 'level': 4, 'score': 0.5052543}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.45130455}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.43942103}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.36090776}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.31003058}, {'id': 'https://openalex.org/C48023723', 'wikidata': 'https://www.wikidata.org/wiki/Q856198', 'display_name': 'Real-time polymerase chain reaction', 'level': 3, 'score': 0.14928195}, {'id': 'https://openalex.org/C190283241', 'wikidata': 'https://www.wikidata.org/wiki/Q14599311', 'display_name': 'Apoptosis', 'level': 2, 'score': 0.09196246}, {'id': 'https://openalex.org/C31573885', 'wikidata': 'https://www.wikidata.org/wiki/Q304484', 'display_name': 'Programmed cell death', 'level': 3, 'score': 0.07810059}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.060741037}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D005434', 'descriptor_name': 'Flow Cytometry', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D050296', 'descriptor_name': 'Microbial Viability', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': True}, {'descriptor_ui': 'D009930', 'descriptor_name': 'Organic Chemicals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D011419', 'descriptor_name': 'Propidium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D013194', 'descriptor_name': 'Staining and Labeling', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D052160', 'descriptor_name': 'Benzothiazoles', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002463', 'descriptor_name': 'Cell Membrane Permeability', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002463', 'descriptor_name': 'Cell Membrane Permeability', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D003951', 'descriptor_name': 'Diagnostic Errors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003959', 'descriptor_name': 'Diamines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004203', 'descriptor_name': 'Disinfection', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004492', 'descriptor_name': 'Edetic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004492', 'descriptor_name': 'Edetic Acid', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D004783', 'descriptor_name': 'Environmental Microbiology', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005434', 'descriptor_name': 'Flow Cytometry', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005456', 'descriptor_name': 'Fluorescent Dyes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D050296', 'descriptor_name': 'Microbial Viability', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009930', 'descriptor_name': 'Organic Chemicals', 'qualifier_ui': 'Q000009', 'qualifier_name': 'adverse effects', 'is_major_topic': False}, {'descriptor_ui': 'D009930', 'descriptor_name': 'Organic Chemicals', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011419', 'descriptor_name': 'Propidium', 'qualifier_ui': 'Q000009', 'qualifier_name': 'adverse effects', 'is_major_topic': False}, {'descriptor_ui': 'D011419', 'descriptor_name': 'Propidium', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011804', 'descriptor_name': 'Quinolines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D045483', 'descriptor_name': 'Rivers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D045483', 'descriptor_name': 'Rivers', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': False}, {'descriptor_ui': 'D012997', 'descriptor_name': 'Solvents', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012997', 'descriptor_name': 'Solvents', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D013194', 'descriptor_name': 'Staining and Labeling', 'qualifier_ui': 'Q000592', 'qualifier_name': 'standards', 'is_major_topic': False}, {'descriptor_ui': 'D013194', 'descriptor_name': 'Staining and Labeling', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013696', 'descriptor_name': 'Temperature', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013997', 'descriptor_name': 'Time Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014871', 'descriptor_name': 'Water Microbiology', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/j.mimet.2016.10.022', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S24124513', 'display_name': 'Journal of Microbiological Methods', 'issn_l': '0167-7012', 'issn': ['0167-7012', '1872-8359'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/27810378', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [{'funder': 'https://openalex.org/F4320324128', 'funder_display_name': 'Rīgas Tehniskā Universitāte', 'award_id': '04000-1.1/17'}], 'datasets': [], 'versions': [], 'referenced_works_count': 47, 'referenced_works': ['https://openalex.org/W137815581', 'https://openalex.org/W1492711335', 'https://openalex.org/W1560562171', 'https://openalex.org/W167202306', 'https://openalex.org/W1691375843', 'https://openalex.org/W1763171946', 'https://openalex.org/W1895129045', 'https://openalex.org/W1965373891', 'https://openalex.org/W1970370189', 'https://openalex.org/W1976836197', 'https://openalex.org/W1982211257', 'https://openalex.org/W1988574274', 'https://openalex.org/W1999769789', 'https://openalex.org/W2000985202', 'https://openalex.org/W2006605582', 'https://openalex.org/W2007969685', 'https://openalex.org/W2008946488', 'https://openalex.org/W2009977841', 'https://openalex.org/W2015808065', 'https://openalex.org/W2018086140', 'https://openalex.org/W2026268643', 'https://openalex.org/W2036379364', 'https://openalex.org/W2040826618', 'https://openalex.org/W2046178807', 'https://openalex.org/W2047409754', 'https://openalex.org/W2052873131', 'https://openalex.org/W2053307446', 'https://openalex.org/W2054473632', 'https://openalex.org/W2058464462', 'https://openalex.org/W2074330765', 'https://openalex.org/W2076095656', 'https://openalex.org/W2077930487', 'https://openalex.org/W2080228586', 'https://openalex.org/W2085436481', 'https://openalex.org/W2097737597', 'https://openalex.org/W2109717868', 'https://openalex.org/W2114783837', 'https://openalex.org/W2115066482', 'https://openalex.org/W2133480990', 'https://openalex.org/W2160199604', 'https://openalex.org/W2164858928', 'https://openalex.org/W2165096119', 'https://openalex.org/W2169823007', 'https://openalex.org/W2171692293', 'https://openalex.org/W2409641876', 'https://openalex.org/W3134463372', 'https://openalex.org/W4249304889'], 'related_works': ['https://openalex.org/W4310481258', 'https://openalex.org/W2594471212', 'https://openalex.org/W2548081959', 'https://openalex.org/W2535513211', 'https://openalex.org/W2383463315', 'https://openalex.org/W2365320098', 'https://openalex.org/W2362126043', 'https://openalex.org/W2036937035', 'https://openalex.org/W2035703912', 'https://openalex.org/W1978847274'], 'abstract_inverted_index': None, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2548081959', 'counts_by_year': [{'year': 2024, 'cited_by_count': 13}, {'year': 2023, 'cited_by_count': 10}, {'year': 2022, 'cited_by_count': 15}, {'year': 2021, 'cited_by_count': 14}, {'year': 2020, 'cited_by_count': 10}, {'year': 2019, 'cited_by_count': 8}, {'year': 2018, 'cited_by_count': 6}, {'year': 2017, 'cited_by_count': 3}], 'updated_date': '2024-12-11T11:06:43.669672', 'created_date': '2016-11-11'}