Title: Simultaneous determination of the residues of four <i>β</i> <sub>2</sub> -agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry
Abstract: An analytical method was developed to simultaneously determine the residues of four β2-agonists in animal foods by high performance liquid chromatography/electrospray-tandem mass spectrometry (HPLC-MS/MS). Samples were extracted with 5% (mass fraction) trichloracetic acid, and then cleaned up by two solid phase extraction cartridges (HLB and ProElut PXC). Quantification of the four β2-agonists was achieved by HPLC-MS/MS in multiple reaction monitoring (MRM) using internal standard method. The limits of detection (S/N=3) of clenbuterol, salbutamol, ractopamine and terbutaline were 0. 05, 0. 05, 0. 05 and 0. 2 µg/kg, and the limits of quantification (S/N= 10) were 0. 25, 0. 25, 0. 1 and 0. 5 µg/kg, respectively. The average recoveries of the four β2-agonists spiked in blank samples at the spiked levels of 2. 5, 5 and 10 µg/kg were 90. 3%-120. 5% with the relative standard deviation (RSD) range of 1. 60%-9. 33%. The method is reliable, sensitive, good recovery and repeatability. It is suitable for the determination of the residues of the four β2-agonists in animal foods.
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 4
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