Title: [Expression of transcription factor T-bet and GATA-3 in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients].
Abstract: Objective To investigate the expression of transcription factors (TF) T-bet and GATA-3 mRNA in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients. Methods Sixty patients were divided into two groups according to the clinical data: group A consisting of stage Ⅰ and Ⅱ, group B including stage Ⅲ and Ⅳ. The gene expression of T-bet and GATA-3 in 60 esophageal cancer patients and 30 healthy controls was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of IFN-γ and IL-4 was measured by enzyme linked immunosorbent assay ( ELISA). Results Expression of T-bet mRNA in esophageal cancer patients( stage Ⅰ and Ⅱ: 0. 27 ±0.05 ng/L, stage Ⅲ and Ⅳ : 0.12 ±0.02 ng/L)was significantly lower than that in the healthy controls ( 1.35 ± 0.14 ng/L) , but the expression of GATA-3 mRNA in esophageal cancer patients ( stage Ⅰ and Ⅱ : 0.45 ± 0.06, stage Ⅲ and Ⅳ : 0.55 ± 0.03 ) was significantly higher than that in the healthy controls ( 0.09 ± 0.10). The plasma level of Th1 cytokine IEN-γ in the patients [ stage Ⅰ and Ⅱ: (12.12 ± 1.48) ng/L, stage Ⅲ and Ⅳ:(8.44 ± 0.90) ng/L] was significantly lower than that in the healthy controls, while the level of Th2 cytokine IL-4 in the patients [ stage Ⅰ and Ⅱ : (18.64 ±0.77) ng/L, stage Ⅲ and Ⅳ: (25.28± 2.02) ng/L] was significantly higher than that in the healthy controls. However, neither in the expression of T-bet and GATA-3, nor in the plasma level of IFN-γ and IL-4, showed a significant difference between group A and B. Conclusion In the peripheral blood of esophageal cancer patients, the expression of T-bet decreased, while GATA-3 increased, Th1/Th2 balance is broken, and the Th2 is dominant. T-bet and GATA-3 play a part role in the regulation of Th1/Th2 balance.
Key words:
Esophageal neoplasms ; T-bet ; GATA-3 ; Th1 ; Th2
Publication Year: 2009
Publication Date: 2009-03-01
Language: en
Type: article
Indexed In: ['pubmed']
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