Title: Determination of Cysteine/Cystine in Gelatin
Abstract: Microcomponents which contain cysteine, cystine and cysteic acid are supposed to be in gelatin. The most active compound seems to be cysteine.Eight lAG-gelatins-bone and hide, lime and aCId treated, oxidized and not oxidized-were investigated by means of HPLC and fluorescence detection. The resulting data provide the sum of cysteine and cystine and by a second determination the sum of cysteine/cystine and cysteic acid content.In spite of the fact that gelatins originate from dIfferent manufacturers it turned out that limed bone gelatins always contain less cysteine/cystine than limed hide gelatins. The same holds for the aCId treated gelatins.In an independent experiment we determined the cysteine content by its specific colour reaction with 5,5'-dithio-bis-(2-nitrobenzoic acid) whereby We also found the difference between oxidized and not oxidized gelatins described above.
Publication Year: 1989
Publication Date: 1989-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 3
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