Title: Alterations in sulphur-containing amino acids in rat brain extracts on storage
Abstract: Difficulties encountered in the amino acid analysis of deproteinised brain tissue extracts are described. Wide variations in some sulphur-containing amino acids occurred as a result of storage at —20°. Increases in the oxidised glutathione and cysteic acid peaks and the appearance of a peak identified as the mixed disulphide of reduced glutathione and cysteine were noted even after 24 h. Attempts have been made to establish the source of cystine molecules which have appeared on storage of the tissue extracts. A simple pH adjustment of the extract to 2.5 before freezing has prevented these changes.
Publication Year: 1973
Publication Date: 1973-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 5
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