Title: [Apoptosis of human gastric cancer cells induced by bcl-2 antisense oligodeoxynucleotides].
Abstract: To study the regulation of bcl-2 gene expression and induction of apoptosis by bcl-2 antisense oligodeoxynucleotides (AS-ODN) on human gastric cancer cell line BGC-823 in vitro.Two bcl-2 AS-ODNs were synthesized, one covering the initiation sequence of translation of bcl-2 mRNA (AS-ODN1) and the other covering the protein coding region (AS-ODN2). BGC-823 cells in logarithmic phase of growth were cultured in the presence of free or liposome (DOTAP)-encapsulated AS-ODN. Cell growth was assessed by MTT method. The expression of bcl-2 at mRNA and protein levels was examined by RT-PCR and flow cytometry, respectively. Electron microscopy and flow cytometry were used to demonstrate apoptotic changes in AS-ODN-treated cells.Both AS-ODNs inhibited proliferation of BGC-823 cells. The inhibitory activity of AS-ODN2 was stronger than that of AS-ODN1. AS-ODNs encapsulated in liposome led to more marked inhibition of cell growth than free AS-ODNs. Both AS-ODNs reduced bcl-2 expression of BGC-823 cells at mRNA and protein levels. Apoptosis of BGC-823 cells were demonstrated by the appearance of apoptotic bodies, chromatin condensation and pre-G1 peak on flow cytometric analysis.Antisense oligodeoxynucleotide of bcl-2 decreases bcl-2 gene expression and induces apoptosis of human gastric cancer cells in vitro.
Publication Year: 1999
Publication Date: 1999-07-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 2
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