Title: [Effect of ginkgolides on gene expression of HIF-1alpha in primary cultured neurons].
Abstract: AIM To study the effects of ginkgolides (Gin) on the expression of hypoxia inducible factor-1 (HIF-1alpha) in hypoxic/ischemic neurons. METHODS The gene expression of HIF-1alpha pretreated with or without Gin (37.5 microg/ml) was observed by RT-PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen-glucose deprivation. RESULTS Some basic expression of HIF-1alpha mRNA were observed in cultured cortical neurons. The expression of HIF-1alpha mRNA increased after 24 h treatment with Gin. The level of HIF-la mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF-1alpha mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin. CONCLUSION Gin could increase the expression of HIF-1alpha mRNA in hypoxic/ischemic cortical neurons.
Publication Year: 2004
Publication Date: 2004-11-01
Language: en
Type: article
Indexed In: ['pubmed']
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