Title: Expression of Hypoxia-Inducible Factor-1α in Cultured Cortical Neurons after Hypoxia-Ischemia
Abstract: Objective To explore the expression of hypoxia-inducible factor-1α(HIF-1α) protein and its mRNA in cultured cortical neurons after hypoxia,ischemia or hypoxia-ischemia(HI) and explore the possibilities of HIF-1α gene therapy in HI neurons.Methods The in vitro models of HI,pure hypoxia and pure ischemia were established using embryonic day 16-18 rats cortical neurons.Immunohistochemical and in-situ hybridization were performed to examine the expression of HIF-1α protein and its mRNA at different reperfusion time points in neurons.Results The expression of HIF-1α protein was very week in normoxic cultured neurons,but was up-regulated while treated with hypoxia and(or) ischemia.HIF-1α expression reached peak at 4 to 8 h after reperfusion with HI,which were statistically significant higher than that at other time points(Pa=0),and decreased gradually at 12 h.Furthermore,HIF-1α protein expression was significantly higher in HI group compared with that in the pure hypoxia or ischemia group(Pa=0).HIF-1α mRNA reached peak immediately after HI,decreased gradually at 2 h,and returned to the baseline at 8 h after reperfusion.Conclusions HIF-1α expression on cortical neurons is regulated differently with hypoxia,ischemia or HI treatment,HIF-1α gene therapy for HI neurons maybe a useful method in the future studies.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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