Title: Single-Cell mRNA Library Analysis by Northern Blot Hybridization
Abstract: The debut of RNA-polymerase cycling reaction (RNA-PCR) has promised to provide linear amplification of a reproducible mRNA library from as few as 20 single cells (1). By incorporating a RNA promoter element during the synthesis of double-stranded complementary DNA (cDNA) templates, a poly(A+) RNA library can be generated and reamplified from the templates in the same conformation and composition as its mRNA origins (Fig. 1). Using microarray analysis, the RNA-PCR-derived poly(A+) library has been proven to contain above 97% of the original poly(A+) RNA population and maintain 88±4% linear correlationship to the populationary ratio of each RNA species ( Chapter 12 ). It has also been tested to generate a full-length mRNA library from as few as 20 homologous tissue cells (2-pg mRNAs) for profiling cancer stages in vivo.
Publication Year: 2003
Publication Date: 2003-11-14
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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