Title: [Effect of alpha-naphthlcetic acid on the proliferation and apoptosis of testis cell].
Abstract: OBJECTIVE: To investigate the effect of alpha-naphthlcetic acid on the proliferation and apoptosis of testis cell. METHODS: Twenty five mice were divided into five groups, 5 mice per group. Basal animal food was fed to the mice in the normal control and positive control groups. Five thousand, 1000, 200 mg/kg of alpha-naphthlcetic acid were added into another three groups. Three days before sacrifice, the mice in positive control group were intraperitoneally injected with cyclophosphamide of 20 mg/kg each day. Four weeks later, all the mice were sacrificed. The proliferation activity of the testis cells was determined by MTT assay, and the levels of Caspase-3 and PCNA protein expression in testis cell were measured by immunohistochemistry method. RESULTS: The testis cell proliferation activity of the high dose group and the normal control group were 0.501 +/- 0.069 and 0.875 +/- 0.082. The difference was significant (P < 0.05). The expression level of PCNA protein and Caspase-3 of the high dose group were 8.9% and 41.3%, respectively, and those of the normal control group were 34.9% and 9.1%. The differences between two groups were significant (P < 0.05). CONCLUSION: alpha-naphthlcetic acid can inhibit the proliferation and induce the apoptosis of testis cells effectively.
Publication Year: 2010
Publication Date: 2010-03-01
Language: en
Type: article
Indexed In: ['pubmed']
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