Title: DDAH2 overexpression inhibits hypoxia-induced apoptosis of rat cardiomyocytes
Abstract: Objective To investigate the inhibitory effect of DDAH2 on the apoptosis induced by hypoxia of rat cardiomyocytes through phosphorylation of eNOS.Methods Cell apoptosis was induced by hypoxia within 36 hours and was determined by wst-1 and flow cytometry.The phosphorylation of eNOS was examined by Western blot.Results Survival rate of hypoxia group was significantly decreased as compared with control group;overexpression DDAH2 induced survival of myocardial cells markedly.After transfection of DDAH2 gene to cardiomyocytes,hypoxia-induced apoptosis significantly declined(17.38%±1.52% vs 27.34%±1.33%,P0.05).DDAH2 gene transfection significantly increased the level of phosphorylation of eNOS in hypoxia of myocardial cells.eNOS inhibitor,L-NAME alleviated the anti-apoptosis effect of overexpression-DDAH2 significantly in H9c2(2-1).Conclusions Overexpression of DDAH2 inhibits the apoptosis of rat cardiomyocytes H9c2(2-1) induced by hypoxia by upregulation of phosphorylation of eNOS.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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