Title: Genotypic resistance mutations to antiretroviral drugs in nave HIV-1 carriers in Beijing
Abstract: Objective To identify the variation of HIV-1strains resistant gene without antiretroviral therapy(ARI)in Beijing area and to give experience on clinical practice.Methods From April to July 2014,601 cases without antiretroviral treatment of HIV infection/AIDS(HIV/AIDS patients)in Beijing were extracted plasma viral RNA,amplified viral pol gene by reverse transcription/nested polymerase chain reaction,and then carried out sequence determination and subtype analysis.It was queried in the Stanford University HIV drug resistance database and analyzed whether there was genotypic resistance and resistant species.Results There were 458 successfully amplified cases,434 were male(94.8%)and 24 were female(5.2%)with average age of 33years(17~77years old).The sequence of primary infection subtype was CRF01_AE(n=211,46.1%),CRF07_BC(n=126,27.5%),B subtype(n=106,23.1%),and C subtype(n=15,3.3%).In the 458 cases,7.4%(34/458)patients suffered primary drug resistance gene mutation,7cases of nucleoside reverse transcriptase inhibitors(NRTIs)resistance,16 cases of non-nucleoside reverse transcriptase inhibitors(NNRTIs)resistance,8cases of protease inhibitors(PIs)resistant,3cases of NRTIs and NNRTIs resistance.Conclusion There were primary drug resistance gene mutation partially in the untreated HIV/AIDS patients in Beijing area.A very few patients were even resistance for two main drugs.Hence,it is necessary to develop drug program according to drug resistance test results before treatment.It will contribute to the improvement of treatment efficiency and prevention of treatment failure.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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