Title: Study of HIV-1 drug-resistance mutation among antiretroviral treatment-nave patients in Zhejiang Province
Abstract: Objective:To obtain background knowledge of drug-resistant mutation among antiretroviral treatment-nave patients in Zhejiang Province,as a supplement for drug resistance surveillance data.Methods:A total of 99 blood samples from antiretroviral therapy-naive patients were collected during 2004~2006.The entire protease gene and 300 amino acids of the reverse transcriptase gene were amplified by RT-PCR and nest-PCR,then sequenced the gene segment.The results were analyzed with HIV db Drug Resistance Algorithm and genotypic resistance mutations were determined to particular anti-HIV drugs.Results:Totally 74 protease gene sequences and 83 reverse transcriptase gene sequences were obtained from patients;1.35% of 73 PR sequences displayed primary resistance mutations in the protease gene,whereas 4.8% of 83 RT sequences have mutations associated with RT inhibitor.The whole rate of drug resistance mutations was about 6.02%.Conclusion:The prevalence of genotypic drug resistance is low in treatment-naive HIV infected patients in this study.The existance of drug resistance mutation can influence the curative effect of antiretroviral therapy.It is important to offer drug resistance mutation observation for individuals before one starts antiretroviral therapy.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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