Title: Detection of hsp mRNA in the tissues of pigs by in situ hybridization
Abstract: According to the gene sequences of hsp70 and hsp90 mRNA,the primers were designed and synthesized.The fragments generated by RT-PCR were cloned into the pGEM-T vector and the recombinant plasmids were identified by PCR amplification.Digoxigenin(DIG) labeled RNA probes were prepared by transcription in vitro in order to establish ISH(in situ hybridization) method.The distributions of hsp70 mRNA and hsp90 mRNA in the tissues of pigs were examined by means of ISH method.Hsp70 mRNA and hsp90 mRNA were found in most of the detected tissues,preferentially in the nuclei and also in the cytoplasm of renal tubular epithelial cells.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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