Title: Establishment and Application of One Step RT-PCR Method for Detection of Newcastle Disease Virus
Abstract:To establish a rapid Newcastle disease virus(NDV) detection method,based on published NDV F genome sequence in GenBank,one pairs of primers were synthesized.Through optimization of the reaction condit...To establish a rapid Newcastle disease virus(NDV) detection method,based on published NDV F genome sequence in GenBank,one pairs of primers were synthesized.Through optimization of the reaction conditions,a one step RT-PCR for detection NDV was established.The method used for infectious bronchitis virus(IBV),infectious bursaldisease virus(IBDV),infectious laryngotracheitis virus(ILTV),all of the PCR results were negative;the sensitivity was 1 ng RNA.Preliminary clinical application showd that the method has strong specificity,high sensitivity,and can be used for the rapid detection of Newcastle disease virus.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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