Title: Establishment of RT-LAMP system for rapid detection of Newcastle disease virus in duck
Abstract:【Objective】 The study was conducted to establish a rapid diagnostic method of reversetranscription loop-mediated isothermal amplification(RT-LAMP) for Newcastle disease virus(NDV) in ducks,and provide...【Objective】 The study was conducted to establish a rapid diagnostic method of reversetranscription loop-mediated isothermal amplification(RT-LAMP) for Newcastle disease virus(NDV) in ducks,and provide supports for the rapid detection of Newcastle disease.【Method】 Six special primers were designed for NDV NP gene with Primer Explorer V4.The RT-LAMP reaction was carried out taking the RNA of Newcastle disease virus SDFCH as its template.The reaction system and conditions were optimized by the sifting of temperature,time and the the concentration of all components.Then we assessed its specificity and sensitivity(take RT-PCR for contrast),and applied it to the inspection of clinical material.【Result】 The Newcastle disease virus(NDV) RNA could be amplified by the optimized RT-LAMP at 63 ℃ within 1h and the amplification products could be observed under naked-eye.The diagnostic method was sensitive and specific,for the amplification results of H9N2 avian influenza virus(AIV),duck reovirns(DRV),Avian metapneumovirus(aMPV) and infectious bronchitis virus(IBV)were negative,and the detection limit of the system was found to be 1×10-3 RNA(0.1 pg/μL) sample,which was 10-fold higher than that of the traditional RT-PCR.The positive rate of twenty-four duck samples suspected newcastle disease was 41.7%.【Conclusion】 The RT-LAMP diagnostic method for Newcastle disease virus(NDV) in ducks was established and it appeared to be fast,accurate,highly sensitive and specific for the purpose.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot