Title: Y chromosome microdeletion and gene deletion screening in infertile men with idiopathic azoospermia and oligospermia
Abstract: Objective: To screen the Y chromosome microdeletion in infertile men with idiopathic azoospermia and oligospermia. Methods: Eighty-nine samples(56 oligospermia and 33 azoospermia patients) were screened for 6 STS loci from AZFa,b and c regions by multi-PCR. The single STS,RBMY1A1 and DAZ gene in those with microdeletion were further verified with single PCR. Results: With the screening and verification,6 infertile men were identified as Y chromosome microdeletion from 89 patients (7%). Among them,AZFc/DAZ deletion occurred in 2 oligospermia and 3 azoospermia men,respectively; another azoospermia man showed a deletion of AZFb+c/DAZ /RBMY1A1. Conclusions: After optimization,89 infertile men were screened and 6 Y chromosome microdeletions were identified. The patients with Y chromosome deletions also had gene deletions. Maybe these genes’ deletion resulted in the male infertility.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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