Title: Analysis on the sensitivity of growth of different type of Vibrio cholera on gentamicin agar plate
Abstract: Objective:To explore the sensitivity of growth of Vibrio cholera of different serotype and toxicogenic ability on gentamicin agar plate.Methods: Double ratio dilution method by physiological saline was applied to different type of Vibrio cholera,and their growth on gentamicin agar plate was observed.Results: The inhibiting capacity of Vibrio cholera on the gentamicin agar plate of factory B was stronger than on which of factory A.The growth condition of Vibrio cholera on the plate of factory A was similar to which on nutrient agar.Imitation inoculation was assayed to compare the growth ability of Vibrio cholera of different serotype.It showed that the minimum growth concentration of Ogawa Vibro cholera on A gentamicin plate was 103 cfu/ml~104 cfu/ml,which on B plate was 105 cfu/ml~106 cfu/ml.The minimum growth concentration of Inaba Vibro cholera on A gentamicin plate was 102 cfu/ml~104 cfu/ml,which on B plate was 104 cfu/ml~105 cfu/ml.The minimum growth concentration of O139 Vibro cholera on A gentamicin plate was 103 cfu/ml~105 cfu/ml,which on B plate was 106 cfu/ml~107 cfu/ml.The minimum growth concentration of CT-NAG on A gentamicin plate was 104 cfu/ml,which on B plate was 107 cfu/ml.Conclusion: Vibrio cholera was inhibited on Gentamicin agar plate,but the inhibiting ability of the gentamicin plate of different factory was different.The growth ability of different Vibrio cholera of the same serotype and same concentration and on the same plate was compared,and it showed which of atoxigenic strain was stronger than toxigenic strain.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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