Title: Optimization of Extraction Technology for Quercitrin from Bark of Carya cathayensis and Determination of Its Content
Abstract: Objective:To optimize extraction technology of quercitrin from bark of Carya cathayensis,and establish its content determination method.Method:Ethanol reflux extraction method was conducted,extraction solution was absorbed by polyamide to remove dopant,then condensed,crystallized and recrystallized to obtain pure product.After identification by NMR,the content of quercitrin was determined by HPLC.Orthogonal test was used to investigate influence of the concentration of ethanol,extraction time and extraction times on yield of quercitrin.Result: NMR verified product as quercitrin,HPLC showed that quercitrin had a good linear relationship in the range of 4.8-91.2 mg·L-1,the content of quercitrin from bark of C.cathayensis was up to 8.37%.Optimum extraction technology was:reflux extracted 2 times with 10 times the amount of 60% ethanol,1 h per time,extraction rate of quercitrin 90%.Conclusion: Established content determination method of quercitrin from C.cathayensis was accurate and simple,optimized extraction technology was stable and feasible.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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