Title: Qualitative identification and extraction technology of bioactive compounds in leaf of Chaenomeles speciosa S.Nakai
Abstract: In order to enhance value in use of Chaenomeles speciosa S.Nakai leaf,the bioactive compounds in leaf of C.speciosa S.Nakai was studied with color and precipitable reaction,and alkaloid,multi-phenolic,saponin were identified.Oleanolic acide(OA) was extracted,separated and purificated with 95% ethanol in orthogonality test.The results showed that the best extraction conditions were the ratio of materials to solution as 1∶12(g·mL-1),extraction time of 6 h,temperature of 60 ℃,extraction 2 times.And the extraction yield was 23.258%.the best extraction and purity conditions were the ratio of materials to solution of 1∶8(g·mL-1),extraction time of 6 h,temperature of 20 ℃,extraction 1 time.And the purity was 1.504%.The results showed that the ratio of materials to solution and extraction time did not have significant effect on the extraction yield,but extraction temperature and times had significant effect on the extraction yield.Meanwhile,the ratio of materials to solution and extraction temperature did not have significant effect on the extraction purity,but extraction times had significant effect on the extraction purity.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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