Title: A preliminary study on the immunoprotection provided by a DNA vaccine of resuscitation-promoting factor D to fight infection in mice
Abstract: Objective To investigate the immunogenicity resulting from a DNA vaccine of resuscitation-promoting factor D of Mycobacterium tuberculosis(RpfD) against H37Rv infection in BALB/c mice.Methods First,90 SPF-grade BALB/c mice were randomly divided into 3 groups(n=30),an RpfD DNA vaccine group,a BCG vaccine group,and a normal saline control group.The RpfD DNA vaccine,BCG,and normal saline were given to mice at 0,3,and 6 weeks.In the ninth week,mice were exposed to aerosolized M.tuberculosis.In the seventeenth week,(1) ELISA was used to test mouse serum and measure the RpfD antibody titer and IFNγ levels,(2) ELISA was used to measure serum-induced IFNγ and IL-12 levels in cultured splenic lymphocytes,(3) CCK-8 was used to determine the lymphocyte proliferation index of murine splenic lymphocytes and LDH was used to measure the CTL activity of lymphocytes,and(4) the bacterial load in mouse lung tissue was determined.Results The RpfD vaccine group had significantly higher levels of RpfD antibodies in serum compared to other groups(P0.01).The RpfD vaccine group also had significantly higher IFNγ levels than the normal saline group(P0.01) and the BCG group(P0.05).The RpfD vaccine group had significantly higher IFNγ levels released by cultured murine splenic lymphocytes in response to in vitro stimulation with RpfD protein compared to other groups(P0.05).Differences in IL-12 levels among the 3 groups were not statistically significant(P0.05).The RpfD vaccine group had significantly higher CCK-8(lymphocyte proliferation index) and LDH(spleen-specific CTL activity) results compared to other groups(P0.05).The RpfD vaccine group had a significantly fewer M.tuberculosis CFUs in lung tissue compared to the other groups(P0.05).Conclusion The RpfD-DNA vaccine induced an immune response in mice that provided immunogenicity.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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