Title: Comparative study on immunogenicity from recombinant BCG to HSP65 DNA vaccine
Abstract: Objective The aim of this study is to evaluate the immunogenicity of recombinant BCG(rBCG) and human Mycobacterium tuberculosis heat shock protein(HSP)65 DNA vaccine. Methods The specific pathogen-free BALB/c mice were immunized with rBCG or HSP65 DNA vaccine by sc or im. The lymphocyte stimulating index(SI), macrophage activity as well as IL-2, IFN-γ levels of the serum and culture supernatant of spleen lymphocyte were tested by the end of 8th week of inoculation. Results After immunization with rBCG vaccine in dose of 10 6 CFU/per capita by sc, the SI was significantly higher than those in the control group, BCG and HSP65 DNA groups ( P 0.05); the macrophage activity and serum IL-2 level were also significantly higher than those in the control group ( P 0.01);the serum IFN-γ level was significantly different from that of HSP65 DNA group ( P 0.05); Compared with each groups. Other parameters were no significantly different( P 0.05). After immunization with HSP65 DNA vaccine in dose of 50μg/per capita by intramuscular injection, the serum IL-2 level was significantly higher than that in the control group ( P 0.01);the SI and serum IFN-γ level were significantly lower than that in the rBCG group( P 0.05);compared with each groups, the macrophage activity and the culture supernatant IL-2 level of spleen lymphocyte were no significantly different( P 0.05). Conclusions The rBCG vaccine may induced stronger immune response in BALB/c mice than did the BCG and HSP65 DNA vaccine, whereas the HSP65 DNA vaccine may induced the immune response of BALB/c mice, but its stimulating effect is lower than that by the rBCG vaccine.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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