Title: Expression of intercellular adhesion molecule 1 mRNA in the anti-inflammatory molecular mechanism of erythromycin
Abstract: Objective To investigate the anti-inflammatory molecular mechanism of erythromycin (EM) in order to find a new way to prevent and treat chronic obstructive pulmonary disease (COPD). Methods Human bronchial epithelial cell line 16HBE was cultured with 10% serum DMEM (high glucose). The cells were randomly divided into eight groups: blank control group,TNF-α (20 ng/ml,16 h),EM(0.3 μg/ml,24 h)+TNF-α (20 ng/ml,16 h),EM(3 μg/ml,24 h)+TNF-α (20 ng/ml,16 h),EM(30 μg/ml,24 h)+TNF-α(20 ng/ml,16 h),EM(0.3 μg/ml,48 h)+TNF-α (20 ng/ml,16 h),EM(3 μg/ml,48 h)+TNF-α(20 ng/ml,16 h),and EM(30 μg/ml,48 h)+TNF-α (20 ng/ml,16 h). Then total cellular RNA was extracted to detect the expression of ICAM-1 mRNA by RT-PCR. The molecular size of ICAM-1 RT-PCR production was not correspondent for what it was designed,therefore,clone sequencing was applied to confirm it. Results ICAM-1 RT-PCR production was ICAM-1 mRNA. ICAM-1mRNA was increased by the addition of TNF-α,which was inhibited by the preincubation with EM in dose-dependent and time-dependent fasion. Conclusions ICAM-1mRNA can be increased by the stimulation of TNF-α,which indicate that EM modify inflammation presumably by suppressing ICAM-1mRNA in human bronchial epithelial cells.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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