Title: Rapid propagation system for tissue culture of Asparagus cochinchinensis
Abstract: Objective Establishing the rapid propagation for plant tissue culture of Asparagus cochinchinensis in order to rationally protect and utilize the medicinal plant resources of A.cochinchinensis.Methods Different media and plant growth regulators at different concentration were used to A.cochinchinensis explants for callus induction,cluster buds induction,and rooting.Results The best medium for callus induction was 1/2 MS + 1.0 mg/L 6-BA + 0.5 mg/L NAA with pH 5.8;The optimal medium for cluster buds induction was MS + 1.5 mg/L 6-BA + 0.5 mg/L IAA with pH 5.8;The optimal medium for rooting was 1/2 MS + 0.5 mg/L IBA + 0.5 mg/L IAA with pH 5.8(iron salt in MS medium reduced to 6.95 mg/L and sucrose decreased to 20 g/L).Conclusion Different explants could be induced for tissue culture and tender bud is the optimal tissue culture explants with the highest induction rate.An effective approach for rapid propagation has been provided,which could promote the artificial cultivation of A.cochinchinensis.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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