Title: Bacillus subtilis B1 Affects Antioxidant Function of Mice Fed a High-Fat Diet
Abstract: The aim of this study was to investigate the effects of Bacillus subtilis B1 on antioxidant function of mice fed a high-fat diet.Forty-five ICR mice with similar weight [(35±1) g] were randomly divided into three groups,control group(fed a basal diet),high-fat group(fed a high-fat diet) and experimental group [fed the high-fat diet supplemented with 0.1%(mass fraction) Bacillus subtilis B1],with 3 replicates per group and 5 mice per replicate.The mice were raised for 30 days to observe the effects of Bacillus subtilis B1 on the antioxidant capacity and oxidative stress in their intestinal mucosa,liver and serum.The results showed as follows: 1) compared with the control group,intestinal mucosal total antioxidant capacity(T-AOC),liver superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT) and thioredoxin reductase(TrxR) activities and serum glutathione(GSH) content,GSH-Px and anti-superoxide anion radical(anti-O-2·) activities in the high-fat group were significantly decreased by 28.34%(P0.01),22.91%(P0.05),37.35%(P0.01),47.47%(P0.01),36.42%(P0.05),30.73%(P0.01),47.26%(P0.01) and 8.02%(P0.01),respectively,whereas liver 8-hydroxydeoxyguanosine(8-OHdG) content in the high-fat group was remarkably increased by 67.87%(P0.01).2) Compared with the high-fat group,intestinal mucosal T-AOC,liver GSH content and CAT activity and serum T-AOC,GSH content,GSH-Px and anti-O-2· activities in the experimental group were significantly increased by 32.44%(P0.05),38.27%(P0.05),53.01%(P0.05),10.72%(P0.05),38.01%(P0.05),51.98%(P0.01) and 13.45%(P0.01),respectively,whereas liver malondialdehyde(MDA) and 8-OHdG contents,and xanthine oxidase(XOD) activity in the experimental group were remarkably reduced by 16.01%(P0.05),37.52%(P0.01) and 16.25%(P0.05),respectively.It is concluded that the high-fat diet can reduce antioxidant capacity and also cause oxidative stress in the intestinal mucosa,liver and serum of mice,and the supplementation of Bacillus subtilis B1 can enhance the antioxidant function of the mice fed the high-fat diet,reduce oxidative stress in their bodies,and protect their cells from oxidative damage.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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