Title: Cows Mycobacterium tuberculosis rpoB and katG Gene Mutations Associated with Multi-drug Resistance
Abstract:The study of cows Mycobacterium tuberculosis rpoB and katG gene mutations and the relation between drug resistance.Take 30 strains of clinical isolates of M.tuberculosis from cattle,use of phage ampli...The study of cows Mycobacterium tuberculosis rpoB and katG gene mutations and the relation between drug resistance.Take 30 strains of clinical isolates of M.tuberculosis from cattle,use of phage amplified biologically assay on the drug sensitivity of Rifampicin and isoniazid,polymerase chain reaction-DNA direct sequencing rpoB and katG gene were amplified and sequenced,analysis of rpoB and katG gene mutations and drug resistance associate.The results showed that 15 resistant strains in four were resistant to RFP,three of them found mutations at condon 531 occurred in 75.00%(3/4);9 single INH-resistant strains of seven mutations,6 isolates mutation at condon 315 occurred in 85.71%(6/7),1 isolate mutation at condon 463 occurred in 14.29%(1/7);2 isolates were resistant to RFP and INH,sequencing showed that two were both mutations at condon 531 in the rpoB gene and condon 315 in the katG gene.All strains were not found the deletion of rpoB and katG gene.The main mechanism of RFP and INH drug resistance is mutations at condon 531 in the rpoB gene and condon 315 in the katG gene;while detection of bovine tuberculosis RFP-resistance,it can be initial screening MDR M.tuberculosis.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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