Title: The effect of Shenfu injection on ischemia reperfusion injury of rat liver graft
Abstract: Objective To investigate the effect of Shenfu injection on ischemia reperfusion injury of rat liver graft. Methods Male SD rats were used as isogeneic orthotopic liver transplantation. Sixty rats were randomly divided into two groups(30 in each). The recipient received Shenfu injection intravenously immediately before the removal of the liver in SF group and normal saline of the same volume in control group. At 3、6 and 24h after the reperfusion blood and hepatic tissue were sampled for examination. Results The levels of superoxide dismutase (SOD) and nitric oxide (NO) in SF group significantly increased compared with those in control group(P0.05). The levels of serum liver enzymes、hyaluronic acid (HA)、malondialdehyde (MDA)、TNF-α、IL-1、endothelin -1( ET-1)and liver cell apoptosis index in SF group were lower than in control group(P0.05). Microscopic examination revealed that the morphological changes of hepatic tissue in control group were more severe than in SF group. ConclusionsShenfu injection has protective effect on ischemia reperfusion injury of rat liver graft. It inhibits the production of oxygen free radical and the activation of Kupffer cells, decreases apoptosis of liver cell and improves microcirculation.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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