Title: Effect of Dahuang Mudan decoction-contained serum on the expression of TLR4 and MyD88 in lipopolysaccharide-stimulated pulmonary macrophages of rats
Abstract: Objective To investigate the therapeutic mechanism of Dahuang Mudan decoction-contained serum( hereinafter referred to as the drug contained serum) in the treatment of sepsis. Methods Mouse pulmonary macrophage cell line RAW264. 7 was cultured in vitro,and then the cells were randomly divided into the blank group,model group,high-, moderate-and low-concentration drug-contained serum groups,each group of 3 holes. Except the blank group,other four groups were stimulated with bacterial lipopolysaccharide( LPS) 5 μg / mL,then were treated with different concentrations of drug-contained serum for 24 h. After the intervention,the cells were cultured in 5% CO2at 37 ℃ for 24 h. Then the mRNA expression of Toll-like receptor 4( TLR4) and myeloid differentiation factor 88( MyD88) was measured by semi-quantitative reverse transcription-polymerase chain reaction( RT-PCR),and their protein expression was analyzed by Western blotting. Results The protein and mRNA expression of TLR4 and MyD88 in the model group was significantly higher than that of the blank group( P 0. 01),but the protein and mRNA expression of TLR4 and MyD88 in the high-and moderateconcentration drug-contained serum groups was significantly lower than that of the model group( P 0. 01),and no significant differences was found between the high- and moderate-concentration drug-contained serum groups. Conclusion The therapeutic mechanism of Dahuang Mudan decoction-contained serum in the treatment of sepsis may be related to the inhibition of TLR4 and down-regulation of MyD88 expression.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 1
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