Title: Influence of Drug Pair of Radix Astragali and Radix Angelicae Sinensis at Different Proportions on Function of OX-LDL Phagocytosis in RAW264.7 Cells
Abstract: Objective To investigate the seropharmacological action of drug pair of Radix Astragali(RA) and Radix Angelicae Sinensis(RAS) at different proportions on function of oxidized low density lipoprotein(OX-LDL) phagocytosis in murine macrophages(RAW264.7).Methods Two experiments were carried out for the study.In the first experiment,6 groups were set up,and they were normal control group,RAW264.7 cultured in serum-free medium,blank serum group,RAW264.7 cultured in the medium with blank serum added and four RA-RAS serum groups,RAW264.7 cultured in the medium with RA-RAS serum which was obtained from SD rats administered RA and RAS at the mass proportions of 1∶ 1,2∶ 1,3∶ 1,5∶ 1(Danggui Buxue Decoction) orally.After co-culture for 24 h,the absorbance value in the 6 groups was detected by methyl thiazolyl tetrazolium(MTT) assay.In the second experiment,7 groups were set up,and they were normal control group,blank serum group,OX-LDL group,and four RA-RAS serum groups.Of them,the treatment of normal control group was as the same as that in experiment one,and RAW264.7 cells in the left 6 groups was pre-cultured with 50 mg / L of OX-LDL for 24h,and then was co-cultured with the corresponding serum for another 24 h.Oil red O staining was applied for detection of lipid phagocytosis by RAW264.7 cells.Results Absorbance value was decreased in four RA-RAS serum groups(P 0.05 or P 0.01),and the results of oil red O staining showed that large amount of lipid droplets were found in RAW264.7 cells of RA-RAS serum groups,and a great quantity of foam cells(macrophages having OX-LDL) were broken,especially in the 5 ∶ 1 RA-RAS(Danggui Buxue Decoction) serum group.Conclusion The therapeutic mechanism of Danggui Buxue Decoction(Radix Astragali and Radix Angelicae Sinensis at the proportion of 5∶ 1) for reducing or eliminating the atherosclerotic plaque is probably related to the inhibition of OXLDL phagocytosis by murine macrophages(RAW264.7).
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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