Title: Tissue Culture and Asexual Line Establishment of Campanula glomerata
Abstract: In order to meet the need for cultivation of germchits,the tender stems with leaves of C.glomerata were used as materials to do the research by using tissue culture methods.The results showed that MS+6-BA 0.6 mg/L + NAA 0.3 mg/L was the optimum medium for differentiation of stems with leaves.The optimum medium for adventitious buds growth was White+GA31.5 mg/L+IAA 0.1 mg/L.After dealt with NAA(concentration of 10 mg/L) for five minutes,the adventitious buds could be easily induced rooting in the medium of 1/3 MS+IAA 0.1 mg/L+NAA 0.2 mg/L.The tube seedlings could be easy to survive after transplanting.Colonization of plantlets grew normally and had good view and admire.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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