Title: The Technical Research on Tissue Culture of Eustoma Grandiforum Shinners
Abstract: [Objective] The aim was to discuss the effects of external hormone on callus induction,uncertain roots differentiation,proliferation and adventitious roots formation of E.grandiforum,to establish manufacturing system of raising seedlings in industrial scale.[Method] Obtaining aseptic plant in the way of aseptic seeding,applying parts of the plant stems as explants,then using randomized experiments to screen the best hormone level for callus formation,uncertain roots differentiation,proliferation and adventitious root formation.[Result]The results show that the suitable culture medium for callus and uncertain roots differentiation of E.grandiforum was MS+BA 0.1-0.5 mg/L+NAA 0.1-0.5 mg/L,the suitable culture medium for proliferation was MS+6-BA 0.1-0.5 mg/L+NAA 0.1-0.5 mg/L+GA 0.1-0.5 mg/L+50-100 mg/L casein,the suitable culture medium root formation was 1/2MS+NAA 0.1-0.3 mg/L or IBA 0.5 mg/L or IAA 0.5 mg/L,which makes the rooting rate reach 97% during 25 days;if the sprouts are transplanted into the substrate in which the rate among ermiculite,perlite and humus soil is 4∶4∶2,the survival can reach over 96%.[Conclusion] Tissue culture technology is used in rapid propagation of E.grandiforum,which has realistic significance for raising seedlings in industrial scale.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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