Title: Effect of interleukin-12,23 subunits in the pathogenesis of myasthenia gravis
Abstract: Objective To explore the effect of interleukin(IL)-12,23 subunits in the pathogenesis of myasthenia gravis (MG).Methods The expression of IL-12p35,IL-23p19 and p40(IL-12/23) mRNA in peripheral blood mononuclear cells (PBMCs) and the levels of serum anti-acetylcholine receptor(AChR)-IgG,interferon-gamma(IFN)-γ and IL-17 were detected by RT-PCR and ELISA in 22 MG patients(including 12 cases of general type and 10 cases of ocular type)and 22 healthy controls,and the correlation among them were analyzed.Results (1) The expression of IL-12p35,IL-23p19 and p40 (IL-12/23) mRNA in PBMCs and the serum IFN-γ and IL-17 concentration in MG group were significantly higher than those in the normal control group (P0.05-0.01); but there was no statistically significant between the MG general type subgroup and MG ocular type subgroup. (2) The serum anti-AChR-IgG were positive in 17 MG patients,and the level was positive correlated with the expression of IL-12p35,IL-23p19,p40 (IL-12/23) mRNA and serum IFN-γ and IL-17 concentrations (r=0.502,0.649,0.817,0.652,0.484;P0.05-0.01). (3) The expression of IL-12p35 mRNA and IL-23p19 mRNA were positive correlated with IFN-γ,IL-17,respectively (r=0.906,0.725;all P0.01). Conclusion IL-12 and IL-23 may participate MG pathogenesis by promoting IFN-γ and IL-17 secretion respectively,but they probably have no correlations with MG type.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot