Title: Determination of Emodin and Chrysophanol in Qingshen Jianfei Tablets by HPLC
Abstract: OBJECTIVE To establish a method for the determination of Emodin and Chrysophanol in Qingshen Jianfei Tablets by HPLC.METHODS Lichrospher C_ 18 column (4.6mm×250mm,5μm)was as stationary phase, Methanol-0.1% phosphoric acid(85∶15)as mobile phase, the temperature of column was set at 30℃ and the wavelength of detection was at 254nm.RESULTS The standard curve of Emodin was rectilinear within the range of 0.03456~0.1728μg,r=1.0000,the average recovery was 99.95%,RSD=0.42%;The standard curve of Chrysophanol was rectilinear within the range of 0.06912~0.3456μg,r=1.0000,the average recovery was 99.19%,RSD=0.96%.CONCLUSION This method is simple and quick, accurate and reproducible.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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