Title: Effects of quercetin on heat shock protein expression in human breast cancer cell MCF-7
Abstract:OBJECTIVE: To investigate the effects of quercetin on heat shock protein expression in human breast cancer cell MCF-7.METHODS: MCF-7 cells were incubated at 42 ℃ for 2 h in vitro.The changes of gene e...OBJECTIVE: To investigate the effects of quercetin on heat shock protein expression in human breast cancer cell MCF-7.METHODS: MCF-7 cells were incubated at 42 ℃ for 2 h in vitro.The changes of gene expression and protein levels of HSP70,HSP27 and HSP90 were detected by real-time PCR and Western-blot after 4,8,12 and 24 hours.MCF-7 cells were pretreated with 150 μmol/L Quercetin before heat treatment,then detected the changes of gene expression and protein levels of HSP70,HSP27 and HSP90.RESULTS: The gene expression and protein levels of HSP70,HSP27 and HSP90 reached to its peak at 4 h,decresed at 8 h and returned to normal level at 24 h after heat treatment.Quercetin could inhibit gene expression and protein levels of HSP70 and HSP27,but up-regulated and delayed descent of HSP90.The level stay in peak at 4 to 8 h and then decrease to normal.CONCLUSIONS: The gene expression and protein levels of HSP70,HSP27 and HSP90 up-regulate quickly after heat treatment.Using quercetin before heat treatment can inhibit gene expression and protein levels of HSP70 and HSP27,but increase and delay that of HSP90.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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