Title: RP-HPLC Determination of Puerarin in Ganmao Qingre Granules
Abstract: Objective:To establish a HPLC method for determination of the content of puerarin in Ganmao Qing re granules.Methods:The condition of RP-HPLC was Suplcosil C 16 column(150 mm×4\^6 mm,5 μm),mobile phase of methanol-water (27∶73)with a flow rate of 1 0 mL·min -1 and UV detector at 248 nm.Results:The calibration curve was linear ( r =0 999 9)within the range of 0 2~200 μg·mL -1 for puerarin,the average recovery was 101 7%( n =3).Conclusion:This method is simple,rapid and reliable,suitable for the quality control of the drug.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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