Title: Determination of chlorogenic acid in Fufang Ganmao Zhike granules by RP-HPLC
Abstract: OBJECTIVE To establish a RP-HPLC method for the determination of chlorogenic acid in Fufang Ganmao Zhike granules.METHODS The chromatographic column of Shim-pack VP-ODS C18(4.6 mm×250mm,5μm)was used,the mobile phase consisting of acetonitrile and 0.2%(V/V)H3PO4 solution(12∶88,V/V);The flow rate was 1.0mL·min-1;the detection wavelength was set at 327 nm and the column temperature was maintained at 30℃.RESULTS Chlorogenic acid showed good linear relationship in the range of the 0.101~0.805 μg(r=0.999.9,n=5),the average recovery was 99.67%,RSD was 1.02%(n=6).CONCLUSION The method is simple,quick,accurate and reliable,and can be used for the determination of this preparation.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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