Title: Effects of Jia-wei-liu-jun-zi Decoction on Cell Cycle and Apoptosis of Human Gastric Carcinoma Cell Line BGC-823 in vitro
Abstract: Objective:To explore the effect of Jia-wei-liu-jun-zi decoction on proliferation and apoptosis of human gastric carcinoma cell line BGC-823 in vitro.Methods:BGC-823 cell line was cultured with 10%,15%,20%,30%,and 40%of Jia-wei-liu-jun-zi decoction in serum,and its proliferation and apoptosis was detected by MMT and flow cytometric,respectively.Results:The survival of BGC-823 cells was decreased after 24,48,72h after culturing with different contents of Jia-wei-liu-jun-zi decoction,and it was correlated with the culturing time and contents.After 24h culturing with 10%,15%,and 20% Jia-wei-liu-jun-zi decoction,the G2 time cell was increased but the S time cell was reduced;the apoptosis rate of the 3 groups was (13.27±0.88)%,(19.97±0.60)%,(33.09±0.58)% as compared with (0.34±0.02)%,(0.19±0.04%),and (0.18±0.03)%,respectively,with a significant difference(P0.01).Conclusion:Jia-wei-liu-jun-zi decoction can inhibit the growth of and may induce the apoptosis of BGC-823 cells.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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