Title: Relationship between Levels of Proliferation and Apoptosis with Clinicopathological Characteristics in Brain Gliomas
Abstract: Objective To investigate the levels of proliferation and apoptosis of brain glioma cells and their relationship to the clinicopathological characteristics.Methods 96 cases of brain gliomas were chosen,and the protein expression of proliferating cell nuclear antigen(PCNA) and apoptosis of tumor cells were investigated respectively by immunohistochemistry method and TdT mediated dUTP nick-end labeling(TUNEL) method.The PCNA label index(PCNA LI) and the apoptosis index(AI) of brain gliomas were calculated.Results The PCNA LI in gliomas of grade Ⅱ,grade Ⅲ and Ⅳ were 26.79±3.45%,44.32±5.83% and 83.31±13.67%,respectively,and higher than that in normal brain tissue.The PCNA LI increased progressively along with grade of gliomas steping up,but AI decreased(P0.05).AI of grade Ⅱ and III tumors were significantly different(P0.05),but there was not any difference between grade III and IV.The negative correlation was suggested between PCNA LI and AI(P0.05).PCNA LI/AI increased progressively along with grade of gliomas steping up.There was an positive correlation between them.Conclusion The overexpression of PCNA protein and inhibition apoptosis may play an important role in the genesis and development of gliomas.Detecting PCNA LI/AI was more significant than doing PCNA LI or AI respectively in determing clinicopathlogic features and malignant behaviors.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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