Title: A Study on in vitro Rapid Propagation Technology for Crisp Plum in Guizhou Province
Abstract: Using the axillary buds as explants for tissue culture,the effects of combinations of plant growth regulators on differentiation,proliferation and rooting of shoots in Crisp Plum were investigated,and the rapid propagation techniques were established.The results showed that the optimal disinfection time of explants in 0.1% HgCl2 was 8 min.The optimal medium for initial culture was WPM+1.0 mg/L 6-BA+0.5 mg/L KT+0.1 mg/L NAA.The optimal multiplication medium was WPM+1.0 mg/L KT+0.1mg/L IBA+1.0 mg/L GA3.The optimal medium for rooting was 1/2MS+0.4 mg/L IBA+1.5%sugar,and its rate for rooting reached 95% and rate of transplanting survival reached 90%.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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