Title: Studies on the tissue culture of Damas 1869 plum
Abstract: Experiment was carried out using the buds of winter shoots advanced by water culture method in room and the spring shoots directly from the field of Damas1869 as material for evaluating the effect of different sterilization on contamination and survival rate in vitro.The effects of different hormones on bud proliferation by 3 factors and 2 levels,the effect of IBA and NAA on rooting were studied.Results showed that the buds of winter shoot were better used as explants than those of spring shoots.The best method for disinfect was washed by 75% alcohol for 10 s and 20% NaClO for 8 min.The survival rate was 64.1%.The optimal medium for shoot generation was MS+6-BA1.0 mg/L+NAA0.5 mg/L+20 g/L sugar+5 g/L agar,the proliferation times could be reached to 7.1.The optimal medium for rooting was 1/2MS+IBA 2.0 mg/L+20 g/L sugar+5 g/L agar+0.05% charcoal,the rooting rate was 87.8%,the average number of roots was 3.9.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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