Title: Protective effects of L-carnitine pretreatment on myocardial ischemia-reperfusion injury of isolated rabbit hearts
Abstract:Objective:To investigate the protective effect of L-carnitine pretreatment against myocardial ischemia-reperfusion (I-R) injury in Langendorff isolated rabbit hearts. Methods:24 rabbit hearts were ran...Objective:To investigate the protective effect of L-carnitine pretreatment against myocardial ischemia-reperfusion (I-R) injury in Langendorff isolated rabbit hearts. Methods:24 rabbit hearts were randomly divided into normal control group, ischemia-reperfusion injury group and L-carnitine group. The normal control group was perfused with Krebs-Henseleit buffer (K-H) solution for 90 min. The I-R injury group was firstly perfused with K-H solution for 30 min, followed by aorta occlusion for 30 min and then reperfused with K-H solution for 60 min. The L-carnitine group was similarly treated to that of the I-R group, except that 10 mmol·L-1 of L-carnitine was added in to the K-H solution before ischemia. The hemodynamic indexes like coronary flow (CF), left ventricular diastolic pressure (LVDP) and it′s first derivative (±dp/dt) were recorded.The activity of malondialdehyde (MDA), superoxide dismutase (SOD), creatine kinase (CK) and lactic dehydrogenase(LDH) in coronary effluent and contents of MDA, SOD and L-carnitine in myocardial tissues were measured in vitro. Results: L-carnitine caused a significant improvement of cardiac function (LVP and ±dp/dtmax increased significantly, and CK enhanced) and a decrease in the release of MDA, LDH and CK in coronary effluent as well as the level of MDA in myocardial tissues, the level of SOD were observed enhanced both in coronary effluent and myocardial tissues. Besides, the content of L-carnitine in muscle was increased (P0.01) and ultramicrostructure indicated a minor injury of heart muscle compared with the I-R injury group (P0.05). Conclusion: L-carnitine pretreatment possesses a protective effect against myocardial I-R injury of isolated rabbit hearts.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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