Title: Protective effects of propofol on myocardium against ischemia-reperfusion injury in isolated rat hearts
Abstract:Objective To investigate the protective effects of different doses of propofol on the myocardium against ischemia-reperfusion (I/R) injury and the possible mechanism. Methods Healthy male SD rats weig...Objective To investigate the protective effects of different doses of propofol on the myocardium against ischemia-reperfusion (I/R) injury and the possible mechanism. Methods Healthy male SD rats weighing 250-300 g were anesthetized with intraperitoneal pentobarbital 40 mg·kg-1. Heparin 1 000 IU·kg-1 was given. The hearts were immediately removed and passively perfused with Lock' s solution aerated with 95 % O2 + 5 % CO2 at 37℃ and constant pressure of 80 cm H2O in a Langendorff apparatus. Forty isolated hearts were divided into 3 groups: control group; I/R group in which the isolated hearts were made globally ischemic for 25 min followed by 30 min reperfusion and propofol group in which the isolated hearts were perfused with perfusate containing propofol 15, 30 or 60 μmol·L-1 before ischemia and during reperfusion. The cardiac function variables including left ventricular developed pressure (LVDP) , the left ventricular end diastolic pressure (LVEDP) ±dp/dtmax, heart rate (HR) , coronary arterial flow (CF) were measured and recorded after equilibrium, before ischemia and at the end of 30 min reperfusion. The coronary outflow was collected and the lactate dehydrogenase (LDH), creatine kinase (CK) activities were measured. At the end of reperfusion a piece of myocardium was obtained. The swelling and activity of and the content of malondialdehyde (MDA) in myocardial mitochondria were determined. The percentage of apoptotic cardiomyocytes was evaluated by flow cytometry and the expression of caspace-3 protein was detected by immuno-histochemistry.Results At the end of 30 min reperfusion LVDP, ±dp/dtmax and CF were significant higher and LVEDP was significantly lower in propofol group (30, 60 μmol· L-1) than in I/R group ( P 0.05 or 0.01). LDH and CK activities in coronary outflow were also significantly lower in propofol group (30, 60 μmol·L-1) than in I/R group ( P 0.05) . Perfusion with propofol 30 or 60 μmol ·L-1 before ischemia and during reperfusion significantly attenuated the increase in mitochondrial swelling and MDA content and the decrease in mitochondrial activity induced by I/R ( P 0.05). The caspace-3 expression and percentage of apoptotic cardiomyocytes were significantly lower in propofol group (30, 60 μmol·L-1) than in I/R group (P 0.05 or 0.01) .Conclusion 30,60 μUmol·L-1 propofol administered before ischemia and during reperfusion has cardioprotective effect. It can improve cardiac function impaired by I/R, attenuate I/R-induced peroxidation injury to mitochondria, down-regulate caspace-3 expression and inhibit cadiomyocyte apoptosis.Read More
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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