Title: Role of CaMKII and p-CREB in pressure overload induced hypertrophic rat hearts
Abstract: AIM To explore the role of calmodolin dependent protein kinase Ⅱ and phosphorylation nuclear transcription factor CREB in the development of myocardial hypertrophy.METHODS The model of hypertensive rats was established by abdominal aortic constriction.CaMKⅡ and pCREB protein expressions of left ventricular tissue were analyzed by immunohistochemistry method.bcl-2 messenger ribonucleic acid(mRNA) expression of left ventricular tissue was analyzed by reverse transcription PCR(RT-PCR) method.RESULTS CaMKⅡ was expressed in both myocardial nuclei myocardial cytoplasmic while pCREB was mainly expressed in myocardial nuclei.The levels of CaMKⅡ and pCREB were significantly higher in cardiac hypertrophy group compared with those in control group.The decrease in bcl-2 mRNA expression was found in cardiac hypertrophy group.CONCLUSION CaMKⅡ and pCREB may be involved in the development of overload induced cardiac hypertrophy.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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