Title: Effects of Dryk1A,ASF and alternative splicing of CaMKIIδ on myocardial hypertrophy in renovascular hypertensive rats
Abstract: AIM:To investigate the role of dual-specificity tyrosine phosporylation-regulated kinase 1A(Dyrk1A)-alternative splicing factor(ASF)-calcium/calmodulin-dependent protein kinase Ⅱδ(CaMK Ⅱδ) pathway in the progression of myocardial hypertrophy in renovascular hypertensive rats.METHODS: The renovascular hypertension was induced by two-kidney one-clip(2K1C) method.The changes of blood pressure and myocardial hypertrophy were measured.The techniques of RT-PCR and Western blotting were used to detect CaMKⅡδ alternative splicing and the protein expression of Dyrk1A and ASF,respectively.RESULTS: Eight weeks after operation,systolic blood pressure(SBP) and diastolic blood pressure(DBP) in 2K1C rats increased(P0.05).The increases in left ventricular weight(LVW),the ratio of LVW to body weight(BW) and the area of myocardial cells indicated that the hypertensive rats developed significant cardiac hypertrophy.The protein expression of Dyrk1A and mRNA expression of CaMKⅡδA and δB were significantly increased,while the protein expression of ASF and mRNA expression of CaMKⅡδC were decreased compared with sham-operated control rats(P0.05).Treatment with Dryk1A inhibitor epigallocatechin gallate(EGCG) or harmine effectively attenuated cardiac hypertrophy and reversed the changes in the protein expression of Dyrk1A,ASF and alternative splicing of CaMKⅡδ(all P0.05).CONCLUSION: Dyrk1A-ASF-CaMKⅡδ pathway plays a role in the development of myocardial hypertrophy in renovascular hypertensive rats.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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