Title: Effects of 17-methoxyl-7-hydroxy-benzfuranchalcon on Intracellular Calcium Overload in Cultured Cardiac Myocytes of Neonatal Rats Injured by H_2O_2 and L-type Calcium Current in Isolated Ventricular Myocytes of Mice
Abstract:Objective: To investigate the effects of 17-methoxyl-7-hydroxy-benzfuranchalcon(YLSC) on intracellular calcium overload in cultured cardiac myocytes of neonatal rats injured by H2O2and L-type calcium ...Objective: To investigate the effects of 17-methoxyl-7-hydroxy-benzfuranchalcon(YLSC) on intracellular calcium overload in cultured cardiac myocytes of neonatal rats injured by H2O2and L-type calcium current(ICa-L) in isolated ventricular myocytes.Method: The cells of primary cultured cardiac myocytes of neonatal rats were divided into groups: ① control group;② model group: administrated with 0.3 mmol.L-1 H2O2;③YLSC treated groups: incubation respectively with 100,200,400 μmol.L-1YLSC for 24 h,and add 0.3 mmol.L-1 H2O2.Laser confocal microscopy was used with Fluo-3 / Am as indicator to detect changes of [Ca2 +]i immediately and 15 mintues after H2O2 intervention.Single ventricular cell was obtained enzymatically by Langendorff perfusion apparatus.The wholecell patch clamp was used to record the ICa-L.Result: ①The average fluorescence intensity values of the model group treated by H2O2 higher significantly than control group and the fluorescence enhancement ratio was 60.43% ± 7.75%.The YLSC could decline the intensity values in a doesdependent manner manner,the fluorescence enhancement ratios of low,middle and high does respectively were 38.39% ± 13.87%,14.49% ± 2.94%,-28.1% ± 1.52%,and all of them were much lower compared with H2O2 group(P 0.01).② YLSC can up-shifted the current-voltage(I-V) curves and markedly shifted the steady state activation and inactivation curve of ICa-L to the left.Conclusion: YLSC inhibited the L-type calcium channel,it can significantly reduce the myocardial intracellular Ca2 + overload induced by H2O2.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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