Title: Effect of naloxone on L-type calcium current in isolated rat ventricular myocytes
Abstract:Objective To evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.Methods Adult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in thi...Objective To evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.Methods Adult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in this study.Single cardiac ventricular myocytes were enzymatically isolated from SD rats.ICa-L was measured in ventricular myocytes and recorded using whole cell patch-clamp technique.Different concentrations (20 and 100 μg/ml) of naloxone were added to the cardiomyocytes.The effect of naloxone on ICa-L was evaluated.Results The peak current of ICa-L Was inhibited by naloxone in a concentration-dependent manner.Naloxone had no significant effect on steady-state activation curve.Conclusion Naloxone inhibits the L-type calcium channel of ventricular myocytes and exerts negative effect on ventricular muscle function.
Key words:
Naloxone; Calcium channels,L-type; Myocytes,cardiacRead More
Publication Year: 2011
Publication Date: 2011-08-20
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot