Title: Content determination of three alkaloids in Coptis chinensis root with RP-HPLC
Abstract: Objective:To determine the content of three alkaloids in Coptis chinensis root with RPHPLC and to test its quality standard.Methods:RP-HPLC was used to test the content of berberine,palmatine,jateorhizine in Coptis chinensis root from Sichuan.The chromatographic column was C18chromatographic column,with acetonitrile-water as mobile phase.Test the peak area and peak time of berberine palmatine,jateorhizine under 345cm,and calculated the content of three alkaloids in the root.Results:the peak area and peak time of berberine,palmatine and jateorhizine were 536.359 31,143.115 22and88.737 28under 345cm.The content of three alkaloids in the root were 5.38%,1.38%and 1.06%.Recovery rate of three alkaloids were 103.8%,103.8%and 117.4%.Precision inspection of Coptis chinensis root was RSD(0.8%).Conclusion:RP-HPLC shows high sensitivity,high precision in determining content of three alkaloids in Coptis chinensis root,indicating it can provide useful evidence for the quality inspection and drug effect evaluation.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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