Title: Determination of Palmatine and Berberine in the Coptis chinensisFranch by High Performance Liquid Chromatography
Abstract: A method for determination of palmatine and berberine from coptis chinensis franch by high performance liquid chromatography(HPLC) was developed. Optimun operating conditions were as follows: ODS C_(18) column. (4.6 250 mm.) CH_(3)CN: 0.05mol/LKH_(2)PO_(4)H_(2)O(pH=2.5)=30∶70(V/V) mobile phase with a flow rate of 0.6 mL/min. and using UV detector at 268 nm. The results of palmatine and berberine show that the calibration equition was C=1.696×10~(-5)A+0.8401, R~(2)=0.9999 and C=1.580×10~(-5)A+0.5114, R~(2)=0.9999. The reproducibility was good with RSD=0.015% and 0.14% respectivelv. The recovery was 99.09%~100.30% and 98.96%~101.86% respectively. The method is accurate, rapid and simple.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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