Title: Cloning and Functional Analysis of an Isopentenyl Diphosphate Isomerase Gene from Maize
Abstract: Isopentenyl diphosphate isomerase(IPI) is the last key enzyme of DXP/MVA pathway in biosynthesis of plant terpenoid precursors.The coding sequence of IPI gene was cloned from Zea mays by reverse-transcription polymerase chain reaction,which was designated as ZmIPI(GenBank accession No:AY738148).The full-length cDNA of ZmIPI is 1 382 bp containing a 1 104 bp open reading frame(ORF) encoding a 367-amino-acid.Color complementation assay indicated that ZmIPI could promote the β-carotene accumulation in engineered XL1-Blue haboring pTrcZmIPI and pAC-BETA,and as a result the engineered bacteria showed the brightly orange given by β-carotene.This suggested that ZmIPI had the typical function of known IPI genes.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot