Title: Full-length cDNA cloning and sequence analysis of isopentenyl diphosphate isomerase involved in saikosaponin biosynthesis pathway of Bupleurum chinense.
Abstract: Objective To clone the full-length cDNA encoding isopentenyl diphosphate isomerase gene(IPPI) associated with saikosaponin biosynthesis pathway in Bupleurum chinense and to provide the basis for further studies on biosynthesis and regulation of saikosaponin.Methods Matrix-based PCR method was used to rapidly screen a B.chinense full-length cDNA library.Results The full-length cDNA of B.chinense IPPI was obtained(GenBank No. gq433719).The length of nucleotide sequence was 1 117 bp and an ORF with 319 amino acids was deduced.Blastx search showed it had the highest similarity to the corresponding protein from Daucus carota subsp.sativus(abb52064) with 92% identity and 97% similarity.Common conserved domains of IPPI were found in B.chinense IPPI including active site,metal binding site,and Nudix motif.A 26 bp long chloroplast signal peptide on the N terminal of the deduced amino acid sequence was predicted by Target P 1.1 and Signal P 3.0.Conclusion The full-length cDNA of B.chinense IPPI is cloned and reported here for the first time.This work will promote the studies on the gene expression pattern and regulatory functions of B.chinense IPPI in saikosaponin biosynthesis.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 2
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